Analytical probing of membranotropic effects of antimicrobial copper nanoparticles on lipid vesicles as membrane models

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dc.contributor.author Izzi, Margherita
dc.contributor.author Oliver, Miquel
dc.contributor.author Mateos, Helena
dc.contributor.author Palazzo, Gerardo
dc.contributor.author Cioffi, Nicola
dc.contributor.author Miró, Manuel
dc.date.accessioned 2023-12-19T08:23:29Z
dc.date.available 2023-12-19T08:23:29Z
dc.identifier.uri http://hdl.handle.net/11201/163191
dc.description.abstract [eng] Copper nanoparticles (CuNPs) are antimicrobial agents that are increasingly being used in several real-life goods. However, concerns are arising about their potential toxicity and thus, appropriate legislation is being issued in various countries. In vitro exploration of the permeability and the distribution of nanoparticles in cell membranes should be explored as the first step towards the investigation of the toxicity mechanisms of metal nanoantimicrobials. In this work, phosphatidylcholine-based large unilamellar vesicles have been explored as mimics of cellular membranes to investigate the effect of ultra-small CuNPs on the physicochemical features of phospholipid membranes. 4 nm-sized CuNPs were synthesized by a wet-chemical route that involves glutathione as a stabilizer, with further characterization by UV-vis absorption spectroscopy, fluorescence spectroscopy, transmission electron microscopy, X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared (FTIR) spectroscopy. Two fluorescent membrane probes bearing naphthalene moieties (laurdan and prodan) were used to monitor the bilayer structure and dynamics, as well as to demonstrate the strong membranotropic effects of CuNPs. The fluorescence spectroscopic studies were supported by dynamic light scattering (DLS) measurements and the calcein leakage assay. Additionally, the degree of perturbation of the phospholipid bilayer by CuNPs was compared against that of Cu2+ ions, the latter resulting in negligible effects. The findings suggested that CuNPs are able to damage the phospholipid membranes, leading to their agglomeration or disruption.
dc.format application/pdf
dc.relation.isformatof https://doi.org/10.1039/D3NA00608E
dc.relation.ispartof Nanoscale Advances, 2023, vol. 5, p. 6533-6541
dc.rights , 2023
dc.subject.classification 54 - Química
dc.subject.other 54 - Chemistry. Crystallography. Mineralogy
dc.title Analytical probing of membranotropic effects of antimicrobial copper nanoparticles on lipid vesicles as membrane models
dc.type info:eu-repo/semantics/article
dc.date.updated 2023-12-19T08:23:29Z
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.identifier.doi https://doi.org/10.1039/D3NA00608E


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