[eng] The Pseudomonas chlororaphis phylogenetic subgroup of species, within the Pseudomonas
fluorescens group, currently includes seven bacterial species, all of which have environmental relevance.
Phylogenomic analyses help clarify the taxonomy of strains in the group and allow for
precise identification. Thirteen antibiotic-resistant strains isolated in a previous study from nine
different sampling sites in the Danube River were suspected to represent a novel species and are
investigated taxonomically in the present study, together with four other strains isolated from the
Woluwe River (Belgium) that were phylogenetically closely related in their rpoD gene sequences.
The strains were characterized phenotypically, chemotaxonomically (fatty acid composition and
main protein profiles), and phylogenetically. They could not be assigned to any known Pseudomonas
species. Three genomes of representative strains were sequenced and analyzed in the context of the
genome sequences of closely related strains available in public databases. The phylogenomic analysis
demonstrates the need to differentiate new genomic species within the P. chlororaphis subgroup and
that Pseudomonas piscis and Pseudomonas aestus are synonyms. This taxonomic study demonstrates
that 14 of the characterized isolates are members of the Pseudomonas_E protegens_A species in the
GTDB taxonomy and that they represent a novel species in the genus Pseudomonas, for which we
propose the name Pseudomonas danubii sp. nov. with strain JDS02PS016T (=CECT 30214T = CCUG
74756T) as the type strain. The other three strains (JDS08PS003, rDWA16, and rDWA64) are members
of the species Pseudomonas_E protegens_B in the GTDB taxonomy and need further investigation
for proposal as a new bacterial species.